The term imouto (literally "little sister" in Japanese) is commonly used in the anime community to refer to, well, little sisters, with the lead male character usually being her big brother.
This page lists all female characters who are second-born or lower, and have older known sibling(s). Foster sisters are also counted.
Other blood relatives (like first cousins) who show the characteristics of an "anime imouto", i.e. having a borderline or outright romantic/sexual attraction to an older male relative are counted here.
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